Datasheet

Bovine Collagen Type V

Name: Bovine Collagen Type V
Brand: Rockland Immunochemicals
Price: 295.0 USD
Availability: InStock

Item No. 001-001-107

Size: 500 µg

Physical State: Liquid (sterile filtered)

Applications: SDS-PAGE

Origin: Bovine

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Specifications for Bovine Collagen Type V

Product Details

Description: Bovine Collagen Type V - 001-001-107

Synonyms: Extracellular Matrix Protein

Species of Origin: Bovine

Type: Native Protein

Target Details

Gene Name: COL5A1/COL5A2

Purity/Specificity: Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure. This product reacts with anti-Collagen Type V. Reaction with Rockland’s anti-Collagen I, II, III, IV or VI is negligible.

Application Details

Tested Applications: SDS-PAGE

Application Note: Bovine COLLAGEN Type V has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Formulation

Concentration: 1.0 mg/mL by nanodrop at 205 nm

Buffer: 0.5 M Acetic Acid

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is six (6) months from date of receipt.

Background

In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.

Frequently Asked Questions (8)

What is the source of the collagen proteins?

Collagen proteins are purified from various sources including bovine or human placenta, skin, nasal tissue, and knee cartilage. The purity of the protein is analyzed by SDS-PAGE.

How are the collagen proteins purified?

The proteins were purified by a proprietary method that includes mechanical separation and salt precipitation. Due to the nature and close association of certain collagen proteins, it is difficult to fully separate each collagen.

In what format are the collagen proteins provided?

Collagen proteins are provided in liquid format at a concentration of around 1 mg/mL. The collagen proteins are solubilized, maintaining their native structure. No enzymatic treatment is performed. The low pH of the buffer is essential in maintaining the solubility of the collagen protein.

In what buffer are the collagen proteins provided?

0.5 M Acetic Acid, pH 2.5 with 0.01% (w/v) Sodium Azide.

What are the recommended SDS-PAGE conditions for collagen proteins?

The recommended buffer for SDS-PAGE is 2X SDS buffer with 8 M Urea. The low pH of the buffer maintains the solubility of the collagen proteins. Also, low-density gels such as 3-8% Tris Acetate should be used. Collagen proteins can be reduced with DTT.

What are the recommended storage conditions for collagen proteins?

Collagen proteins should always be stored at 4°C. It is important to never let the collagen proteins freeze because this will degrade the protein. Please be sure to maintain 4°C temperature during assays such as SDS-PAGE.

How was the concentration determined?

The concentration of the collagen proteins was measured by absorbance via nanodrop at 205 nm. When measuring concentration, please be sure to blank with the same buffer as the protein product (0.5 M Acetic Acid).

Are collagen proteins suitable for cell culture?

Since no testing for endotoxin or other contaminants was performed, we do not recommend the use of this product for cell culture.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.