AKT1 (phosphatase treated) Human Recombinant Protein
AKT1 (phosphatase treated) Human Recombinant Protein SDS-Page
AKT1 (phosphatase treated) Human Recombinant Protein Western Blot
Western Blot of Rabbit AKT Antibody.
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Datasheet

AKT1 (phosphatase treated) Human Recombinant Protein

009-001-I51
10 µg
Liquid
WB, SDS-PAGE
Human
$315.00 /Per Item
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Product Details

AKT1 (phosphatase treated) Human Recombinant Protein - 009-001-I51
RAC, PKB, AKT, PKB alpha, PRKBA
Human
Recombinant Protein

Target Details

AKT1  - View All AKT1 Products
Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
P31749 - UniProtKB
62241011 - NCBI Protein
207 - Gene ID

Application Details

SDS-PAGE, WB
Human AKT1 (phosphatase treated) recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Cell Line Data

None (Control)

Formulation

1mg/ml by UV absorbance at 280 nm
20 mM Tris pH8, 300 mM NaCl with 10% glycerol
None
10% (v/v) Glycerol

Shipping & Handling

Dry Ice
Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiration date is one (1) year from date of receipt.
AKT1 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and RAC (Related to A and C kinases). AKT is a key regulator of many signal transduction pathways, and it exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT1 recombinant protein is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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