ZBP-89 Antibody
Anti-ZBP-89 Antibody - Western Blot
Schematic representation of the ZBP-89 and p53 interaction domains.
Anti-ZBP-89 Antibody - Western Blot
Schematic diagram of ZBP-89 protein domains and function.
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Datasheet

ZBP-89 Antibody

Rabbit Polyclonal

100-401-685
100 µL
Liquid (sterile filtered)
WB, ELISA
Human
Rabbit
$475.00 /Per Item
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Product Details

Anti-ZBP-89 (RABBIT) Antibody - 100-401-685
rabbit anti-ZBP-89 Antibody, Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody, Zinc finger protein 148, Transcription factor ZBP-89, ZNF148
Rabbit
Polyclonal
Antiserum

Target Details

ZNF148  - View All ZNF148 Products
Human
Recombinant Protein
Purified full length ZBP-89 recombinant protein expressed in E.coli.
Anti-ZBP89 antibody was prepared from monospecific antiserum by delipidation and defibrination. This polyclonal antibody is specific for human ZBP-89.  Reactivity with ZBP-89 from other species has not been determined.
Q9UQR1 - UniProtKB
CAA15422.1 - NCBI Protein
256711 - Gene ID

Application Details

ELISA, WB
This polyclonal antibody reacts with human ZBP-89 in a variety of tested immunological assays including western blot and ELISA.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Formulation

90 mg/mL by Refractometry
None
0.1% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store ZBP89 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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