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Vinculin Antibody
Rabbit Polyclonal
600-401-FW4
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Vinculin (RABBIT) Antibody - 600-401-FW4
Vinculin Antibody, MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV
Rabbit
Polyclonal
IgG
Target Details
VCL - View All VCL Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Vinculin antibody was prepared from whole rabbit serum produced by repeated immunizations with a 23 amino acid peptide near the internal region of human Vinculin.
Anti-Vinculin antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.
Application Details
ELISA, IF, IHC, WB
Anti-Vinculin Antibody has been tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 124 kDa in Western Blots of specific cell lysates and tissues.
Formulation
1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
