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Vimentin Antibody
Mouse Monoclonal 2D1 IgG2a
200-301-E54
100 µL
Liquid (sterile filtered)
WB, IHC
Human, Mouse, Rat, Bovine
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Vimentin (MOUSE) Monoclonal Antibody - 200-301-E54
Vim, Vimentin Antibody
Mouse
Monoclonal
IgG2a
Target Details
VIM - View All VIM Products
Human, Mouse, Rat, Bovine
Recombinant Protein
Anti-Vimentin Antibody was produced in mice by repeated immunizations with a recombinant human vimentin purified from E. coli.
Anti-Vimentin Antibody was purified from tissue culture supernatant by protein G chromatography.
Application Details
IHC, WB
Anti-Vimentin Antibody is tested for Western Blot, immunocytochemistry, and immunohistochemistry, and it is specific for the ~50 kDa vimentin protein. Specific conditions for reactivity should be optimized by the end user.
Formulation
Titrated value sufficient to run approximately 10 mini blots.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Expiration date is one (1) year from date of receipt.
Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin Antibody is ideal for researchers interested in neuroscience research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
