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Urease Antibody
Rabbit Polyclonal
200-4182
200-4182S
200-4182-0100
50 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Lyophilized
WB
Jack Bean
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Urease (Jack Bean) (RABBIT) Antibody (BULK ORDER) - 200-4182
rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
Rabbit
Polyclonal
IgG
Target Details
UREA_CANEN - View All UREA_CANEN Products
Jack Bean
Native Protein
Urease [Jack Bean]
Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
P07374 - UniProtKB
AAA83831.1 - NCBI Protein
Application Details
WB
Anti-Urease Antibody has been tested in western blot and suitable against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Urease catalyzes the hydrolysis of urea to produce ammonia and carbamate (ammonia and carbonic acid). Ureases are found in numerous bacteria, fungi, algae, invertebrates, plants, in soils, as a soil enzyme. Due to the catalytic activity producing ammonia the pH of the environment increases. Ureases, when bacterial, can be a mode for the pathogens of many medical conditions such as hepatic encephalopathy, hepatic coma, urinary stones, and peptic ulcers. Urea is found naturally in the environment as well as artificially introduced. Anti-Urease antibody is ideal for researchers interested in ecosystems, microbes, and gut bacteria.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
