Datasheet

Urease Antibody

Name: Urease Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 200-4182

Size: 50 mg

Physical State: Lyophilized

Applications: WB

Reactivity: Jack Bean

Host Species: Rabbit

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50 mg $0.00

25 µL $125.00

100 µg $475.00

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Specifications for Urease Antibody

Product Details

Description: Anti-Urease (Jack Bean) (RABBIT) Antibody (BULK ORDER) - 200-4182

Synonyms: rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: UREA_CANEN - View All UREA_CANEN Products

Reactivity: Jack Bean

Immunogen Type: Native Protein

Immunogen: Urease [Jack Bean]

Purity/Specificity: Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.

Database Links:

P07374 - UniProtKB

AAA83831.1 - NCBI Protein

Application Details

Tested Applications: WB

Application Note: Anti-Urease Antibody has been tested in western blot and suitable against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Formulation

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Urease catalyzes the hydrolysis of urea to produce ammonia and carbamate (ammonia and carbonic acid). Ureases are found in numerous bacteria, fungi, algae, invertebrates, plants, in soils, as a soil enzyme. Due to the catalytic activity producing ammonia the pH of the environment increases. Ureases, when bacterial, can be a mode for the pathogens of many medical conditions such as hepatic encephalopathy, hepatic coma, urinary stones, and peptic ulcers. Urea is found naturally in the environment as well as artificially introduced. Anti-Urease antibody is ideal for researchers interested in ecosystems, microbes, and gut bacteria.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.