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UBC12 Antibody
Rabbit Polyclonal
2 References
600-401-865
100 µg
Liquid (sterile filtered)
WB, ELISA
Human, Mouse, Rat, Chimpanzee, Dog, Frog
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Human UBC12 (RABBIT) Antibody - 600-401-865
rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody
Rabbit
Polyclonal
IgG
Target Details
UBE2M - View All UBE2M Products
Human, Mouse, Rat, Chimpanzee, Dog, Frog
Conjugated Peptide
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region near aa 150-183 of Human UBC12 protein.
UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human UBC12 protein. However, 100% homology is on record for this protein from human, chimpanzee, frog, mouse, rat and dog as indicated by BLAST analysis. Reactivity with UBC12 proteins from other sources is not known.
Application Details
ELISA, WB
UBC12 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.
Formulation
0.73 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.
Liu et al. (2018). Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Molecular Cell
Applications
WB, IB, PCA
Kolokoltsova OA et al. (2008). Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling. Virology.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.