Datasheet

TRPC6 Antibody

Name: TRPC6 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-H29

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA, IHC, IF

Reactivity: Human, Mouse

Host Species: Rabbit

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Specifications for TRPC6 Antibody

Product Details

Description: Anti-TRPC6 (RABBIT) Antibody - 600-401-H29

Synonyms: TRP6

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: TRPC6 - View All TRPC6 Products

Reactivity: Human, Mouse

Immunogen Type: Conjugated Peptide

Immunogen: TRPC6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide near the n-terminus of human TRPC6.

Purity/Specificity: Anti-TRCP6 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with TRCP6 with Human and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.

Database Links:

Q9Y210 - UniProtKB

AAH93658 - NCBI Protein

7225 - Gene ID

Application Details

Tested Applications: ELISA, IF, IHC, WB

Application Note: Anti-TRPC6 Antibody is tested for use in E, WB, IF, and IHC. Expect a band approximately ~106.3 kDa on specific lysates. Western Blot, Immunocytochemistry, and Immunofluorescence tested in human samples; Immunohistochemistry in mouse samples. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

TRPC6 antibody detects human TRPC6. The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP” (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, TRPC6 acts as a non-selective store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that TRPC6 encodes endogenous DAG-activated receptor-operated cation channels in vivo. Anti-TRPC6 antibodies are ideal for investigators involved in enzyme research.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Sandwich ELISA Protocol for Collagen

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.