Datasheet

TLR8 Antibody

Name: TLR8 Antibody
Brand: Rockland Immunochemicals
Price: 549.0 USD
Availability: OutOfStock

Mouse Monoclonal 44C143 IgG1 kappa

Item No. 200-301-I30

Size: 100 µg

Physical State: Liquid

Applications: WB, IHC, IF, FC, Dot Blot

Reactivity: Human

Host Species: Mouse

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Specifications for TLR8 Antibody

Product Details

Description: Anti-TLR8 (MOUSE) Monoclonal Antibody - 200-301-I30

Synonyms: Toll-like receptor 8

Host Species: Mouse

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: TLR8 - View All TLR8 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: TLR8 Antibody was produced in mice prepared by repeated immunizations with amino acids corresponding to an internal sequence of human TLR8 protein.

Purity/Specificity: Anti-TLR8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR8 from human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR8 from other sources has not been determined.

Database Links:

Q9NR97 - UniProtKB

NP_619542.1 - NCBI Protein

gene?db=gene&term=51311 - Gene ID

Application Details

Tested Applications: Dot Blot, FC, IF, IHC, WB

Application Note: Anti-TLR8 Antibody is tested for use in WB, DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, and IHC-P. Expect a band approximately 121kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 0.5 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.05% (w/v) Sodium Azide

Stabilizer: 0.05% BSA

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-TLR8 CD288 Antibody detects human TLR8. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa. Anti-TLR8 CD288 Antibody is ideal for investigators involved in cytokines and growth factor research.

Related Protocols

Adherent Cell Lysis Protocol

Flow Cytometry (FC) Protocol

Fluorescent Western Blotting Protocol

General Immunoblotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.