Datasheet

TET2 Antibody

Name: TET2 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-FC9

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA

Reactivity: Human, Mouse

Host Species: Rabbit

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Specifications for TET2 Antibody

Product Details

Description: Anti-TET2 (RABBIT) Antibody - 600-401-FC9

Synonyms: TET2 Antibody, MDS, KIAA1546, Nbla00191, Methylcytosine dioxygenase TET2

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: TET2 - View All TET2 Products

Reactivity: Human, Mouse

Immunogen Type: Conjugated Peptide

Immunogen: Anti-TET2 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the C-terminus of human TET2.

Purity/Specificity: Anti-TET2 antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. TET2 antibody is human and mouse reactive. At least two isoforms of TET2 are known to exist; this antibody will detect the larger isoforms. This antibody is predicted to not cross-react with TET1 and TET3.

Database Links:

Q6N021 - UniProtKB

NP_001120680.1 - NCBI Protein

54790 - Gene ID

Application Details

Tested Applications: ELISA, WB

Application Note: Anti-TET2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 224 kDa in Western Blots of specific cell lysates and tissues.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

TET2, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. It is a candidate tumor suppressor gene reported to be mutated in approximately 14% of patients with JAK2V617F-positive myeloproliferative neoplasms (1), and can be mutated in other hematopoietic disorders such as myelodysplastic syndromes, acute myeloid leukemia, and chronic myelomonocytic leukemia (2). Analysis of the TET2 and JAK2 mutations in these neoplasms suggests that mutations in TET2 do not represent a predisposition for acquiring mutations in JAK2.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.