TCRbeta Fluorescein Antibody
Flow Cytometry of anti-TCRbeta FITC - 200-B02-N92
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Datasheet

TCRbeta Fluorescein Antibody

Hamster Armenian Monoclonal H57-597 IgG

200-B02-N92
500 µg
Liquid (sterile filtered)
FC
Mouse
Hamster Armenian
$365.00 /Per Item
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Product Details

Anti-TCRbeta (Armenian Hamster) Fluorescein Conjugated Monoclonal Antibody - 200-B02-N92
Tib, TCRbeta, T cell receptor beta chain, Tcrb
Hamster Armenian
Fluorescein (FITC)
Monoclonal
IgG

Target Details

Tcrb
Mouse
Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.
Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta. Cross reactivity with TCRbeta from other sources has not been tested. Anti-TCRbeta is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
?term=21577 - Gene ID

Application Details

FC
Anti-TCR beta is tested for FLOW and useful for Immunofluorescence and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Formulation

0.5mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Expiration date is six (6) months from date of receipt.
The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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