T7 RNA Polymerase Antibody
ELISA results of Rabbit Anti-T7 RNA Polymerase Antibody
Western Blot Results of Rabbit Anti-T7 RNA Polymerase Antibody
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Datasheet

T7 RNA Polymerase Antibody

Rabbit Polyclonal

200-401-MX5
50 µg
Liquid (sterile filtered)
WB, ELISA
Virus
Rabbit
$475.00 /Per Item
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Product Details

Anti-T7 RNA Polymerase (RABBIT) Antibody - 200-401-MX5
Escherichia phage T7 (Bacteriophage T7), DNA-directed RNA polymerase, DNA-dependent RNA polymerase, RNA nucleotidyltransferase (DNA-directed)
Rabbit
Polyclonal
IgG

Target Details

T7p07  - View All T7p07 Products
Virus
Recombinant Protein
Anti-T7 Polymerase antibody was produced from whole rabbit serum produced by repeated immunizations with T7 Polymerase isolated from E. coli.
Protein A purified T7 RNA Polymerase antibody is directed against T7 Polymerase (E. coli). The product was purified from monospecific antiserum by protein A chromatography. 
entry - UniProtKB
NP_041960.1 - NCBI Protein
1261050 - Gene ID

Application Details

ELISA, WB
This protein A purified Anti-T7 RNA Polymerase antibody has been tested for use in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 98.9 kDa in size corresponding to T7 Polymerase by western blotting in the appropriate cell lysate or extract.

Formulation

1.19 by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
T7 RNA Polymerase is a highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. It recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. T7 RNA Polymerase unwinds the double-stranded DNA to expose the coding strand for templating. It participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also, T7 RNA Polymerase plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process. In eukaryotes three forms of this enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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