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T4 RNA Ligase Antibody
Rabbit Polyclonal
200-401-MX4
50 µg
Liquid (sterile filtered)
ELISA
Virus
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-T4 RNA Ligase (RABBIT) Antibody - 200-401-MX4
Enterobacteria phage T4, RNA ligase 1, polyribonucleotide synthase (ATP), ribonucleic ligase
Rabbit
Polyclonal
IgG
Target Details
rnlA - View All rnlA Products
Virus
Recombinant Protein
Anti-T4 RNA Ligase antibody was produced from whole rabbit serum produced by repeated immunizations with T4 Ligase isolated from E. coli.
Protein A purified T4 RNA Ligase antibody is directed against T4 RNA Ligase (E. coli). The product was purified from monospecific antiserum by protein A chromatography.
Application Details
ELISA
This protein A purified Anti-T4 RNA Ligase antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43.5 kDa in size corresponding to RNA Ligase by western blotting in the appropriate cell lysate or extract.
Formulation
1.19 by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The T4 RNA Ligase enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA. This catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[RNA]. Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in the formation of the phosphodiester bond and release of the adenylate.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.