Western blot of Synapsin I Ser9 Antibody

Datasheet

Synapsin I phospho S9 Antibody

Name: Synapsin I phospho S9 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 612-401-C93

Size: 100 µL

Physical State: Liquid

Applications: WB

Reactivity: Mouse, Rat

Host Species: Rabbit

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Specifications for Synapsin I phospho S9 Antibody

Product Details

Description: Anti-Synapsin I pS9 (RABBIT) Antibody - 612-401-C93

Synonyms: Synapsin I, SYN1, Synapsin-1

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: Syn1 - View All Syn1 Products

Reactivity: Mouse, Rat

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Synapsin I pS9 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of Synapsin I.

Purity/Specificity: Anti-Synapsin I pS9 antibody is directed against rat Synapsin I. The antibody detects the phosphorylated S9 amino acid. Anti-Synapsin I pS9 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase. Cross-reactivity is expected from the following species based on 100% sequence homology: bovine, human, mouse, Xenopus and zebra fish.

Database Links:

P09951 - UniProtKB

NP_001104252.1 - NCBI Protein

Application Details

Tested Applications: WB

Application Note: Anti-Synapsin I pS9 (Rabbit) antibody is tested for use in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 78 kDa in size corresponding to synapsin I doublet protein phosphorylated at Ser9 in the appropriate cell lysate or extract. This antibody also weakly labels the approximately 55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Researchers should determine optimal titers for applications that are not stated below.

Formulation

Concentration: Titrated value sufficient to run approximately 10 mini blots.

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Synapsin I pS9 antibody is directed against rat Synapsin I, which plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth. Anti-Synapsin I pS9 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.