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Datasheet

Sucrose Phosphorylase Antibody Peroxidase Conjugated

Goat Polyclonal

200-103-231 200-103-231S 200-103-231-0100
20 mg 25 µL 100 µg
Lyophilized Liquid (sterile filtered) Lyophilized
E. coli
Goat
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$125.00 /Per Item
$455.00 /Per Item
20 mg $0.00
25 µL $125.00
100 µg $455.00
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Product Details

Anti-Sucrose Phosphorylase (E. coli) (GOAT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-103-231
goat anti-Sucrose Phosphorylase Antibody, HRP Conjugated goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase
Goat
Peroxidase (HRP)
Polyclonal
IgG

Target Details

ycjM  - View All ycjM Products
E. coli
Native Protein
Sucrose Phosphorylase [E.coli]
Sucrose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli].  Cross reactivity against Sucrose Phosphorylase from other sources is unknown.
P76041 - UniProtKB
YP_852475.1 - NCBI Protein
4492194 - Gene ID

Application Details

Anti-Sucrose Phosphorylase has been assayed against 1.0 µg of Sucrose Phosphorylase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Sucrose Phosphorylase is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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