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Datasheet

Sarcosine Oxidase Antibody Peroxidase Conjugated

Goat Polyclonal

200-103-093 200-103-093S 200-103-093-0100
20 mg 25 µL 100 µg
Lyophilized Liquid (sterile filtered) Lyophilized
WB, ELISA
Sarcosine Oxidase (Microbial)
Goat
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$125.00 /Per Item
$455.00 /Per Item
20 mg $0.00
25 µL $125.00
100 µg $455.00
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Product Details

Anti-Sarcosine Oxidase (Microbial) (GOAT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-103-093
goat anti-Sarcosine Oxidase Antibody, HRP Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit
Goat
Peroxidase (HRP)
Polyclonal
IgG

Target Details

soxA  - View All soxA Products
Sarcosine Oxidase (Microbial)
Native Protein
Sarcosine Oxidase [Microbial]
Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].  Cross reactivity against Sarcosine Oxidase from other sources is unknown.
Q46337 - UniProtKB
YP_004446819.1 - NCBI Protein
10586229 - Gene ID

Application Details

ELISA, WB
Anti-Sarcosine Oxidase has been tested by ELISA and western blot and is assayed against 1.0 ug of Sarcosine Oxidase [Microbial] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Formulation

10 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Sarcosine oxidase is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide, and 5,10-methylenetetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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