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Rhodopsin Antibody
Mouse Monoclonal 1D4 IgG1
200-301-E29
100 µL
Liquid
WB, IHC
Amphibians, Mammalian
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Rhodopsin (MOUSE) Monoclonal Antibody - 200-301-E29
OPN2, Rhodopsin, RHO, Anti-Rhodopsin monoclonal, photoreceptor proteins
Mouse
Monoclonal
IgG1
Target Details
RHO - View All RHO Products
Amphibians, Mammalian
Native Protein
Anti-Rhodopsin Monoclonal Antibody was produced in mouse by repeated immunizations with purified native bovine rhodopsin.
Anti-Rhodopsin antibody is directed against bovine Rhodopsin. Rhodopsin antibodies are Protein G purified from cell culture supernantant. Reactivity is predicted to human and mouse based on strong homology. Cross reactivity from other sources has not been determined.
P02699 - UniProtKB
Application Details
IHC, WB
Anti-Rhodopsin antibody has been tested in Western Blot and Immunohistochemistry. Expect a band of approximately 39 kDa in size corresponding to the rhodopsin proteins in Western blot in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
Formulation
Titrated value sufficient to run approximately 10 mini blots.
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Rhodopsin antibody detects rhodopsin which is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis. Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin. Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding. Anti-Rhodopsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
