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RFX5 Antibody
Rabbit Polyclonal
4 References
200-401-193
100 µg
Liquid (sterile filtered)
WB, ELISA, ChIP, EMSA
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-RFX5 (N-terminal) (RABBIT) Antibody - 200-401-193
rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody
Rabbit
Polyclonal
IgG
Target Details
RFX5 - View All RFX5 Products
Human
Conjugated Peptide
RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 55 kDa and 85 kDa.
Application Details
ELISA, WB
ChIP, EMSA
- View References
This product was assayed by immunoblot and found to be reactive against RFX5 (N-terminal specific) from a variety of fibroblast and B-cell lysates at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-RFX5 (N-terminal specific) is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.
Chen, DB et al. (2020). RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A. Scientific Reports
Applications
ChIP
Stavride P et al. (2013). Differential regulation of MHCII genes by PRMT6, via an AT-hook motif of RFX5. Mol Immunol.
Applications
ChIP
Brickey WJ et al. (1999). Analysis of the defect in IFN-gamma induction of MHC class II genes in G1B cells: identification of a novel and functionally critical leucine-rich motif (62-LYLYLQL-68) in the regulatory factor X 5 transcription factor. J Immunol.
Applications
WB, IB, PCA
Gobin SJ et al. (1998). The RFX complex is crucial for the constitutive and CIITA-mediated transactivation of MHC class I and β2-microglobulin genes. Immunity.
Applications
Supershift, Gel Shift, EMSA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
