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REDD1 Phospho T23/25 Antibody

Name: REDD1 Phospho T23/25 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-X24

Size: 100 µL

Physical State: Liquid (sterile filtered)

Applications: WB

Reactivity: Human

Host Species: Rabbit

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Specifications for REDD1 Phospho T23/25 Antibody

Product Details

Description: Anti-REDD1 pT23/25 (RABBIT) Antibody - 600-401-X24

Synonyms: DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: DDIT4 - View All DDIT4 Products

Reactivity: Human

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Phospho-Thr23/25 REDD1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1 conjugated to KLH.

Purity/Specificity: Anti-REDD1 pT23/pT25 Antibody is directed against REDD1 protein phosphorylated at T23 and T25. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated REDD1. Reactivity against REDD1 occurs from human sources. However, reactivity is also expected against non-human primate based on 100% sequence homology.

Database Links:

Q9NX09 - UniProtKB

NP_061931.1 - NCBI Protein

54541 - Gene ID

Application Details

Tested Applications: WB

Application Note: Anti-Phospho-Thr23/25 REDD1 Antibody is tested for Western Blots and is specific for the ~34 kDa REDD1 phosphorylated at Thr23/25. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: Titrated value sufficient to run approximately 10 mini blots.

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Preservative: None

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiration: Expiration date is one (1) year from date of receipt.

Background

REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli. REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress. It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b. REDD1 pT23/25 Antibody is ideal for researchers interested in cancer research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.