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Ready-To-Use SMAD3 Antibody
Rabbit Polyclonal
600-401-E70-RTU
100 µL
Liquid (sterile filtered)
WB
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Ready-To-Use Anti-SMAD3 (RABBIT) Antibody - 600-401-E70-RTU
hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody, Smad-3
Rabbit
Polyclonal
IgG
Target Details
SMAD3 - View All SMAD3 Products
Human
Conjugated Peptide
SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
RTU Anti-SMAD3 Antibody is directed against human Smad3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against human Smad3 protein corresponding to an internal region surrounding amino acid residue 179. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Application Details
WB
Ready-To-Use Anti-SMAD3 Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-SMAD3 (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract. Although not tested, this antibody is likely functional in ELISA, immunoprecipitation, and immunohistochemistry. Optimal titers for these applications should be determined by the researcher.
Formulation
sufficient to run 10 immunoblot tests
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
0.01% Bovine Serum Albumin (BSA), 25% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
Expiration date is one (1) year from date of receipt.
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response. All Rockland Immunochemical’s SMAD3 antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
