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Datasheet

RBM35B Antibody

Rabbit Polyclonal

600-401-DX1
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human, Mouse
Rabbit
$475.00 /Per Item
Out of stock
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Anti-RBM35B (RABBIT) Antibody - 600-401-DX1
RBM35B Antibody, RBM35B, RBM35B, PP7059, Epithelial splicing regulatory protein 2, RNA-binding motif protein 35B
Rabbit
Polyclonal
IgG

Target Details

ESRP2  - View All ESRP2 Products
Human, Mouse
Conjugated Peptide
Anti-RBM35B antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the C-terminus of human RBM35B.
Anti-RBM35B Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least three isoforms of RBM35B are known to exist; this antibody will detect all three.
Q9H6T0 - UniProtKB
NP_079215 - NCBI Protein
80004 - Gene ID

Application Details

ELISA, IF, IHC, WB
Anti-RBM35B Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 78 kDa in Western Blots of specific cell lysates and tissues.

Formulation

1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programmer induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.