Western Blot of Anti-Pyruvate Kinase Antibody

Datasheet

Pyruvate Kinase Antibody

Name: Pyruvate Kinase Antibody
Brand: Rockland Immunochemicals
Availability: InStock
Rating: 5 (2 reviews)

Goat Polyclonal

2 References

Item No. 200-1178

Size: 50 mg

Physical State: Lyophilized

Applications: WB

Reactivity: Rabbit

Host Species: Goat

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50 mg $0.00

25 µL $125.00

100 µg $475.00

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Specifications for Pyruvate Kinase Antibody

Product Details

Description: Anti-Pyruvate Kinase (Rabbit Muscle) (GOAT) Antibody (BULK ORDER) - 200-1178

Synonyms: goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Host Species: Goat

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: PKM - View All PKM Products

Reactivity: Rabbit

Immunogen Type: Native Protein

Immunogen: Pyruvate Kinase (Rabbit Muscle)

Purity/Specificity: Anti-Pyruvate Kinase (Rabbit Muscle) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle). Anti-Pyruvate Kinase (Rabbit Muscle) Antibody has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.

Database Links:

P11974 - UniProtKB

NP_001182574.1 - NCBI Protein

100008676 - Gene ID

Application Details

Tested Applications: WB

Application Note: This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Pyruvate Kinase is an enzyme involved in the glycolytic process that transfers a phosphate group from phosphoenolpyruvate onto ADP, yielding energy in the form of ATP and a pyruvate molecule. The isoforms of pyruvate kinase are of special interest in biology. Normal tissues utilize the m1 isoform of pyruvate kinase which creates and transports pyruvate to create ATP within the cells mitochondria. However, the m2 isoform of pyruvate kinase is prevalent in tumors, causing lower levels of pyruvate production. Study of the two isoforms and their relative activity and expression is becoming integral to understanding tumor proliferation, especially glioblastomas. Anti-Pyruvate Kinase (Rabbit Muscle) Antibody is ideal for investigators in Cancer, Cell Biology, and Neuroscience.

References (2)

Meabon J et al. (2016). Intracellular LINGO-1 negatively regulates Trk neurotrophin receptor signaling. Molecular and Cellular Neurosciences

Applications

WB, IB, PCA

PubMed

Meeker K et al. (2015). Partial Loss of the Glutamate Transporter GLT-1 Alters Brain Akt and Insulin Signaling in a Mouse Model of Alzheimer's Disease. Journal of Alzheimer's Disease : Jad

Applications

WB, IB, PCA

PubMed

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Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.