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Datasheet

PTEN-P1 Antibody

Rabbit Polyclonal

600-401-A90S 600-401-A90
25 µL 100 µg
Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA
Human
Rabbit
$125.00 /Per Item
$475.00 /Per Item
25 µL $125.00
100 µg $475.00
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In stock
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Anti-PTEN-P1 (RABBIT) Antibody - 600-401-A90
rabbit anti-PTEN-P1 antibody, Phosphatase and tensin homolog 2, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1
Rabbit
Polyclonal
IgG

Target Details

PTEN  - View All PTEN Products
Human
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.
This affinity-purified antibody is directed against human PTEN protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Reactivity occurs against human PTEN protein.   A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse, dog sources based on 82% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
O43460 - UniProtKB
73765544 - NCBI Protein
5728 - Gene ID

Application Details

ELISA, WB
This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.

Formulation

1.15 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
This gene (PTENP1) is a highly homologous pseudogene of PTEN.  PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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