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POL II Antibody
Rabbit Polyclonal
1 References
600-401-HB3S
600-401-HB3
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
ELISA, ChIP
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-POL II (RABBIT) Antibody - 600-401-HB3
RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A,
Rabbit
Polyclonal
IgG
Target Details
POLR2A - View All POLR2A Products
Human
Conjugated Peptide
Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH).
This affinity purified antibody is directed against human POL II. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and Chinese hamster.
Application Details
ChIP, ELISA
Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-POL II antibody will detect DNA-directed RNA polymerase II subunit RPB1, an enzyme composed of 12 subunits, which initiates the transcription of RNA from DNA, synthesizing many functional non-coding RNAs as well as messenger RNA precursors. POL II encodes a core element of the transcription machinery. Malfunctioning of the Pol II gene can cause diseases such as Cockayne Syndrome B and La Crosse Encephalitis. Anti-POL II Antibody is useful for researchers interested in DNA damage and repair studies.
Serresi M et al. (2021). Functional antagonism of chromatin modulators regulates epithelial-mesenchymal transition. Sci Adv.
Applications
ChIP
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
