Datasheet is currently unavailable. Try again or CONTACT US
PIN1 Antibody
Rabbit Polyclonal
4 References
600-401-A20
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-PIN1 (RABBIT) Antibody - 600-401-A20
rabbit anti-PIN! antibody, PIN-1, PIN 1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody, Peptidyl-prolyl cis-trans isomerase Pin1, Prolyl isomerase antibody, Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody, Protein NIMA interacting 1 antibody, Rotamase Pin1 antibody, PPIase Pin1 antibody
Rabbit
Polyclonal
IgG
Target Details
PIN1 - View All PIN1 Products
Human
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1.
This affinity purified antibody is directed against human Pin1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.
Application Details
ELISA, IHC, WB
This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to Pin1 by western blotting in the appropriate cell lysate or extract. Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.
Formulation
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Pin1 (peptidylprolyl cis/trans isomerase NIMA-interacting 1 protein) is an essential peptidylprolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond and also catalyzes pSer/Thr-Pro cis/trans isomerizations. Pin1 shows a nuclear localization.
Lucchetti C et al. (2013). The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer. PLoS One
Applications
WB, IB, PCA
Rizzolio F et al. (2013). Dissecting Pin1 and phospho‐pRb regulation. J Cell Physiol.
Applications
Undefined
Rizzolio F et al. (2012). Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death Differ.
Applications
WB, IB, PCA
La Montagna R et al. (2012). Androgen receptor serine 81 mediates Pin1 interaction and activity. Cell Cycle
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.