Phosphotyrosine Antibody Biotin Conjugated
Rabbit Polyclonal
600-406-262
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IP
Phosphotyrosine
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-PhosphoTyrosine (pY) (RABBIT) Antibody Biotin Conjugated - 600-406-262
PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit
Rabbit
Biotin
Polyclonal
IgG
Target Details
Phosphotyrosine
Other
Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Anti-PhosphoTyrosine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphothreonine or phosphoserine.
Application Details
ELISA, IHC, IP, WB
Anti-PhosphoTyrosine antibody is tested for use in ELISA, immunohistochemistry-P, IP, and western blotting assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA and flow cytometry.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store Anti-pY at -20° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Anti-PhosphoTyrosine detects phosphorylated Tyrosines. Tyrosine is one of the 22 amino acids that are used by cells to synthesize proteins. Tyrosine has a phenol group. It functions as a receiver of phosphate groups that are transferred by way of protein kinases. Phosphorylation of the hydroxyl group changes the activity of the target protein. Anti-PhosphoTyrosine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.