Phosphoserine Antibody
Anti-PhosphoSerine (pS) Antibody - Western Blot
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Phosphoserine Antibody

Rabbit Polyclonal

1 References
600-401-261
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IP
Mouse
Rabbit
Phosphoserine Antibody Peroxidase Conjugated Phosphoserine Antibody Biotin Conjugated
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Product Details

Anti-PhosphoSerine (pS) (RABBIT) Antibody - 600-401-261
pS antibody, phospho serine AB, PhosphoSerine (pS) reactive antibody, proteinogenic amino acid antibodies, Phosphoserine [pS] polyclonal antibody, PhosphoSerine antibody produced in rabbit
Rabbit
Polyclonal
IgG

Target Details

Mouse
Other
Phosphoserine Antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.
Anti-Phosphoserine Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.  Reactivity is specific for phosphoserine and minimal cross-reactivity is observed against phosphotyrosine or phosphothreonine.

Application Details

ELISA, IHC, IP, WB
Phosphoserine Antibody is tested for use in ELISA, western blotting, Immunohistochemistry-P, and immunoprecipitation assays.  The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues.  The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA and flow cytometry.

Formulation

0.25 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
None
50% (v/v) Glycerol

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Phosphoserine Antibody detects phosphorylated Serines. Serine is one of the proteinogenic amino acids. By virtue of the hydroxyl group, serine is classified as a polar amino acid. It is not essential to the human diet, since it is synthesized in the body from other metabolites, including glycine. Serine is important in metabolism in that it participates in the biosynthesis of purines and pyrimidines. It is the precursor to several amino acids including glycine and cysteine, and tryptophan in bacteria. It is also the precursor to numerous other metabolites, including sphingolipids and folate, which is the principal donor of one-carbon fragments in biosynthesis. Anti-phospho Serine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.
(). The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling. J Cell Biol.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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