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PAK 1 Phospho T84 Antibody
Rabbit Polyclonal
600-401-X21
100 µL
Liquid (sterile filtered)
WB
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-PAK-1 pT84 (RABBIT) Antibody - 600-401-X21
Serine/Threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, p65-PAK
Rabbit
Polyclonal
IgG
Target Details
PAK1 - View All PAK1 Products
Human, Mouse, Rat
Phosphorylation
Conjugated Peptide
Anti-Phospho-Thr84 PAK1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr84 of human PAK1 conjugated to KLH.
Anti-Phospho-Thr84 PAK1 Antibody is directed against PAK1 protein phosphorylated at T84. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated PAK1. Minimal reactivity occurs against non-phosphorylated PAK1. Reactivity against PAK1 occurs from human sources. However, reactivity is also expected against bovine, canine, chicken, non-human primates, sheep, and zebra fish based on 100% sequence homology.
Application Details
WB
Anti-Phospho-Thr84 PAK1 Antibody is tested for Western Blots and is specific for the ~68 kDa PAK1 phosphorylated at Thr84. Specific conditions for reactivity should be optimized by the end user.
Formulation
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
None
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Expiration date is one (1) year from date of receipt.
PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation. Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif. OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin. PAK1 pT84 Antibody is ideal for researchers interested in cell cycle research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
