Anti-p53 (ac Lys305) (RABBIT) Antibody - Western Blot

Anti-p53 (ac Lys305) (RABBIT) Antibody- Dot Blot

Datasheet

p53 K305 Ac Antibody

Name: p53 K305 Ac Antibody
Brand: Rockland Immunochemicals
Price: 125.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-GW2

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, Dot Blot

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

25 µL $125.00

100 µg $475.00

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Specifications for p53 K305 Ac Antibody

Product Details

Description: Anti-p53 (ac Lys305) (RABBIT) Antibody - 600-401-GW2

Synonyms: rabbit anti-p53 Ac-Lys305 antibody, rabbit anti-p53 K305ac antibody, cellular tumor antigen p53, tumor suppressor p53, phosphoprotein p53, Antigen NY-CO-13, TP53

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: TP53 - View All TP53 Products

Reactivity: Human

PTM Specificity: Acetylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-p53 K305 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 305 of human p53.

Purity/Specificity: Anti-p53 K305 Ac antibody is directed against human p53 protein. p53 K305 Ac Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.

Database Links:

P04637 - UniProtKB

NP_000537.3 - NCBI Protein

7157 - Gene ID

Application Details

Tested Applications: Dot Blot, WB

Application Note: p53 K305 Ac Antibody has been tested by dot blot and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.6 kDa in size corresponding to p53 by western blotting in the appropriate cell lysate or extract.

Formulation

Concentration: 1.0 mg/ml by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

TP53 (tumor suppressor gene p53) is one of the most well-studied genes that suppresses tumor formation and renders protection against DNA damage by inducing cell cycle arrest, DNA repair, or apoptosis. TP53 signaling is triggered through numerous cellular events ranging from DNA damage to hypoxia, stress and a plethora of other causes. Upon activation, p53 acts as zinc-containing transcriptional regulator and initiates a cascade of events that determines the cellular outcome including cell cycle arrest, apoptosis, senescence, DNA repair, development, differentiation and tissue homeostasis. Cell cycle arrest is induced by p53 via trans-activating genes such as p21 (CDK-inhibitor 1, cyclin dependent kinase) and others. Interestingly, p53 itself is capable of triggering cellular responses (survival or induced cell death) as well. Mutations or deletions in the TP53 gene are present in nearly 50% of human cancers, and primarily results in impaired tumor suppressor function. Anti-p53 (ac Lys305) antibody is ideal for researchers interested in developmental biology, cell growth and cancer research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

General Immunoblotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.