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p27 kip1 phospho S140 Antibody
Rabbit Polyclonal
1 References
600-401-K08S
600-401-K08
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, ELISA
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-p27 kip1 pS140 (RABBIT) Antibody - 600-401-K08
rabbit anti-p27 kip1 pS140 antibody, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Kip-1, KIP1, CDKN1B
Rabbit
Polyclonal
IgG
Target Details
CDKN1B - View All CDKN1B Products
Human
Phosphorylation
Conjugated Peptide
Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
Anti-p27 kip1 pS140 is directed against the phosphorylated form of human p27 kip1 protein at the S140 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. This antibody will specifically react with a p27kip1 protein from human tissue. Reactivity against p27 from other species is unknown.
Application Details
ELISA, WB
Anti-p27kip1 pS140 antibody is tested for use in ELISA, immunoprecipitation, co-immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.
Formulation
1.23 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
p27 kip1 pS140 is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Phosphorylation occurs on serine, threonine and tyrosine residues. Anti-p27 kip1 pS140 antibody is ideal for researchers interested in cell cycle and cancer research.
Mishra, SK et al. (2019). Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells. Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
