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p19Arf Antibody
Rat Monoclonal 5.C3.1 IgG2b
1 References
200-501-891
100 µg
Liquid (sterile filtered)
WB, ELISA, IF
Human, Mouse, Hamster
Rat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-p19Arf (exon 2) (RAT) Monoclonal Antibody - 200-501-891
rat anti-p19Arf Antibody, Cyclin-dependent kinase inhibitor 2A isoform 3, p19 ARF
Rat
Monoclonal
IgG2b
Target Details
Cdkn2a - View All Cdkn2a Products
Human, Mouse, Hamster
Conjugated Peptide
This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 54-75 of mouse p19Arf protein.
This is a Protein G purified antibody directed against mouse p19Arf protein. No reactivity is observed against human or hamster homologues. The epitope was putatively mapped to amino acids 62-75 of mouse p19Arf. BLAST analysis indicates that no significant sequence homology exists for this sequence with p19Arf homologues from other sources. The homologue from rat only shows 11 of 14 identities (78% homology). No additional information is available for reactivity with p19Arf protein from other sources.
Application Details
ELISA, IF, WB
This antibody has been tested for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to mouse p19Arf by western blotting in the appropriate cell line or lysate.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The Ink4a-Arf locus encodes two tumor suppressor proteins, p16Ink4a and p19Arf. Expression of Ink4a and Arf is regulated by distinct promoters upstream of alternative first exons whose products are spliced to a common second exon translated in alternative reading frames (from which Arf gets its name). The two genes are induced by different stress signals and can be separately mutated or silenced in tumor cells. Targeted disruption of either or both genes in mouse leads to spontaneous tumor formation, which is accelerated by exposure of the mutant animals to chemical carcinogens or ionizing radiation. Inactivation of ARF by mutation, deletion, or epigenetic silencing is observed in many human cancers, underscoring the role of this protein as a potent and ubiquitous tumor suppressor. p19Arf is activated by Myc or mutant Ras. Once activated, p19Arf binds to the p53 negative regulator Mdm2, leading to p53 stabilization and unleashing a p53-dependent transcriptional program that triggers either cell cycle arrest or apoptosis. p19Arf also has p53-independent inhibitory effects on cell proliferation. p19Arf is a nucleolar protein and is implicated in ribosomal biogenesis.
Michalak et al. (2018). Canonical PRC2 function is essential for mammary gland development and affects chromatin compaction in mammary organoids. PLOS Biology
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
