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ORAI1 Antibody
Rabbit Polyclonal
1 References
600-401-DG9
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF, FC, IP
Human, Mouse
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-ORAI1 (RABBIT) Antibody - 600-401-DG9
ORAI1 Antibody, IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1
Rabbit
Polyclonal
IgG
Target Details
ORAI1 - View All ORAI1 Products
Human, Mouse
Conjugated Peptide
Anti-ORAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human ORAI1.
Anti-ORAI1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Application Details
ELISA, IF, IHC, WB
FC, IP
- View References
Anti-ORAI1 Antibody has been tested for use in ELISA, Western Blotting, immunofluorescence, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 33 kDa in Western Blots of specific cell lysates and tissues.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.
Carreras-Sureda A et al. (2021). S-acylation targets ORAI1 channels to lipid rafts for efficient Ca2+ signaling by T cell receptors at the immune synapse. eLife.
Applications
FC, FACS, FLOW; IP, Co-IP; WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
