Datasheet is currently unavailable. Try again or CONTACT US
Nuclease/NucA Antibody
Rabbit Polyclonal
200-401-MU6
100 µg
Liquid (sterile filtered)
WB, ELISA
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Nuclease/NucA (RABBIT) Antibody - 200-401-MU6
Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA
Rabbit
Polyclonal
IgG
Target Details
nucA - View All nucA Products
Recombinant Protein
Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.
This antibody was purified from monospecific antiserum by Protein A chromatography. This antibody will react with endonuclease from S. marcescens. Cross reactivity from other sources is unknown.
P13717 - UniProtKB
Application Details
ELISA, WB
Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.06 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35°C and 44°C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.