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NRF1 Antibody
Rabbit Polyclonal
5 References
200-401-869
500 µg
Liquid (sterile filtered)
WB, ELISA, IHC, Other
Human, Mouse
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-NRF1 (RABBIT) Antibody - 200-401-869
rabbit anti-NRF1 antibody, rabbit anti-NRF 1 antibody, NRF-1, alpha pal antibody, alpha palindromic binding protein antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody
Rabbit
Polyclonal
IgG
Target Details
Nrf1 - View All Nrf1 Products
Human, Mouse
Recombinant Protein
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.
This protein A purified antibody is directed against mouse NRF1. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with NRF1 homologues from other sources has not been determined.
Application Details
ELISA, IHC, WB
Other
- View References
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
Formulation
2.2 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.
Hinkey JM et al. (2019). Exercise training prevents doxorubicin-induced mitochondrial dysfunction of the liver. Med Sci Sports Exerc.
Applications
WB, IB, PCA
Das JK et al. (2018). NRF1-mediated oncogenic reprogramming drives estrogen-induced breast carcinogenesis. Cells.
Applications
WB, IB, PCA
Miura et al. (2017). Mitochondrial-Targeted Antioxidant Maintains Blood Flow, Mitochondrial Function, and Redox Balance in Old Mice Following Prolonged Limb Ischemia. International Journal of Molecular Sciences
Applications
WB, IB, PCA
Joseph AM et al. (2014). Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats. Biogerontology.
Applications
WB, IB, PCA
Nguyen LMD et al. (2014). Effect of near-infrared light exposure on mitochondrial signaling in C2C12 muscle cells. Mitochondrion.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.