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Nox1 Antibody
Rabbit Polyclonal
600-401-R15
100 µg
Lyophilized
WB, IHC
Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-NOX1 (RABBIT) Antibody - 600-401-R15
NADPH oxidase 1, NOX-1, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, Nox1, Mox1, Noh1
Rabbit
Polyclonal
IgG
Target Details
Nox1 - View All Nox1 Products
Mouse, Rat
Conjugated Peptide
NOX1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of rat NOX1.
Anti-NOX1 antibody is directed against rat NOX1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and mouse based on homology for the immunogen sequence. Cross reactivity with NOX1 from other sources has not been determined.
Application Details
IHC, WB
Anti-NOX1 is tested in Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65.2 kDa corresponding to the appropriate cell lysate or extract.
Formulation
0.5 mg/ml by UV absorbance at 280 nm
0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
0.05mg Thimerosal
5mg BSA
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion. This antibody is suitable for researchers interested in epigenetic antibodies and cancer research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.