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Nitrotyrosine Antibody
Mouse Monoclonal 39B6 IgG2a
200-301-A98
100 µg
Liquid (sterile filtered)
WB, IHC
Broad
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Nitrotyrosine (MOUSE) Monoclonal Antibody - 200-301-A98
Protein tyrosine nitration, 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid, Nitro tyrosine Antibody, 3-Nitrotyrosine Antibody
Mouse
Monoclonal
IgG2a
Target Details
Broad
Native Protein
This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate.
This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues. This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources. Reactivity with nitrotyrosine from other sources is also expected.
Application Details
IHC, WB
This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 0.7 µg of antibody is sufficient to detect 5 µg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).
Formulation
1.0 mg/mL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophil peroxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation-induced tissue injury (1). The presence of nitro tyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis (4).
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.