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Ngp Antibody
Rabbit Polyclonal
1 References
600-401-GW9S
25 µL
Liquid (sterile filtered)
WB, IF
Mouse
Rabbit
This product is discontinued
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Product Details
Anti-NGP (Neutrophilic granule protein) (RABBIT) Antibody - 600-401-GW9S
rabbit anti-NGP antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein
Rabbit
Polyclonal
IgG
Target Details
Ngp - View All Ngp Products
Mouse
Recombinant Protein
Affinity purified Anti-NGP antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse NGP protein.
Anti-NGP is directed against full-length mouse NGP. This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in NGP based on 100% homology match for the immunogen sequence.
Application Details
IF, WB
This affinity purified antibody has been tested for use in IF and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to NGP by western blotting in the appropriate cell lysate or extract.
Formulation
1.10 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer’s research.
Hong J et al. (2019). Neutrophilic granule protein is a novel murine LPS antagonist. Immune Netw.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.