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NF-Y Antibody
Rabbit Polyclonal
6 References
200-401-100
100 µg
Liquid (sterile filtered)
WB, IHC, IF, ChIP, EMSA
Human, Monkey
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-NF-Y (A subunit) (RABBIT) Antibody - 200-401-100
rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A
Rabbit
Polyclonal
IgG
Target Details
NFYA - View All NFYA Products
Human, Monkey
Conjugated Peptide
Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
Application Details
WB
ChIP, EMSA, IF, IHC
- View References
Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Formulation
0.60 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.
Falcone, E et al. (2016). Infinity: An In-Silico Tool for Genome-Wide Prediction of Specific DNA Matrices in miRNA Genomic Loci. PloS One
Applications
ChIP
Gurtner, A. et al. (2008). NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue. PloS One
Applications
IHC, ICC, Histology; ChIP; IF, Confocal Microscopy
Gialitakis M et al. (2006). Coordinated changes of histone modifications and HDAC mobilization regulate the induction of MHC class II genes by Trichostatin A. Nucleic cids Res.
Applications
ChIP
Grimm M et al. (2002). Inhibition of Major Histocompatibility Complex Class II Gene Transcription by Nitric Oxide and Antioxidants. J Biol Chem.
Applications
WB, IB, PCA
Gobin SJ et al. (2001). The MHC-specific enhanceosome and its role in MHC class I and β2-microglobulin gene transactivation. J Immunol.
Applications
Supershift, Gel Shift, EMSA
Westerheide SD et al. (1999). Orientation and positional mapping of the subunits of the multicomponent transcription factors RFX and X2BP to the major histocompatibility complex class II transcriptional enhancer. Nucleic cids Res.
Applications
Supershift, Gel Shift, EMSA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
