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Neurofilament H Antibody
Mouse Monoclonal NAP4 IgG1
900-301-D82
100 µL
Liquid
WB, IHC
Rat, Bovine, Chicken
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Neurofilament H (MOUSE) Antibody - 900-301-D82
mouse anti-Neurofilament H Antibody, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Mouse
Monoclonal
IgG1
Target Details
NEFH - View All NEFH Products
Rat, Bovine, Chicken
Native Protein
Anti-NF-H Antibody was produced by repeated immunizations with purified native bovine NF-H.
Anti-NF-H antibody is directed against Neurofilament H. The antibody is protein G purified from concentrated clarified cell culture supernate. Anti-NF-H recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. The antibody has been directly tested for reactivity in chicken and a wide variety of mammals. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
P12036 - UniProtKB
DAA20547.1 - NCBI Protein
Application Details
IHC, WB
Anti-Neurofilament H antibody is tested for use in Western Blotting, IHC, and ICC. Specific . Expect a band of approximately 200 kDa in size corresponding to the Neurofilament H proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
NF-H antibody detects Neurofilament H. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-ANeurofilament H antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
