Datasheet is currently unavailable. Try again or CONTACT US
MYC Epitope Tag Antibody
Rabbit Polyclonal
9 References
600-401-381
100 µg
Liquid (sterile filtered)
WB, ELISA, IF
Myc-Tag
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Myc Epitope Tag (RABBIT) Antibody - 600-401-381
Rabbit anti-MYC Epitope Tag Antibody, Rabbit anti-c-myc, Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu
Rabbit
Polyclonal
IgG
Target Details
Myc-Tag
Conjugated Peptide
This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.
This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Details
ELISA, WB
IF
- View References
Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody was tested by ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Chen Y et al. (2023). Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5. Cell Rep.
Applications
WB, IB, PCA
Cao X et al. (2021). Alt-RPL36 downregulates the PI3K-AKT-mTOR signaling pathway by interacting with TMEM24. Nat Commun.
Applications
WB, IB, PCA
Chang CW et al. (2020). Pathogenic mutations reveal a role of RECQ4 in mitochondrial RNA: DNA hybrid formation and resolution. Sci Rep.
Applications
WB, IB, PCA
Tanga N et al. (2019). The PTN‐PTPRZ signal activates the AFAP1L2‐dependent PI3K‐AKT pathway for oligodendrocyte differentiation: Targeted inactivation of PTPRZ activity in mice. Glia.
Applications
WB, IB, PCA
Liu, W et al. (2019). Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope. Journal of Molecular Cell Biology
Applications
WB, IB, PCA
Yue et al. (2018). SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death & Disease
Applications
WB, IB, PCA
Wojcik J et al. (2016). Allosteric inhibition of Bcr-Abl kinase by high affinity monobody inhibitors directed to the Src homology 2 (SH2)-kinase interface. J Biol Chem.
Applications
WB, IB, PCA
Fujikawa et al. (2015). Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5. PLOS One
Applications
WB, IB, PCA
Sorenson EB et al. (2010). γ‐Secretase‐Dependent Cleavage Initiates Notch Signaling from the Plasma Membrane. Traffic.
Applications
IF, Confocal Microscopy; WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.