Rockland anti mouse IL-17A monoclonal antibody - Flow Cytometry

Anti Mouse IL-17A Antibody - Western Blot

Datasheet

Mouse IL-17A Antibody

Name: Mouse IL-17A Antibody
Brand: Rockland Immunochemicals
Price: 125.0 USD
Availability: InStock

Rat Monoclonal 20B4.G10.F5 IgG2a kappa

Item No. 210-501-B32

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA, FC, Multiplex

Reactivity: Mouse

Host Species: Rat

$485.00 /Per Item

25 µL $125.00

100 µg $485.00

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Specifications for Mouse IL-17A Antibody

Product Details

Description: Anti-IL-17A (RAT) Monoclonal Antibody - 210-501-B32

Synonyms: rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

Host Species: Rat

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: IL17a - View All IL17a Products

Reactivity: Mouse

Immunogen Type: Recombinant Protein

Immunogen: This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.

Purity/Specificity: This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.

Database Links:

Q62386 - UniProtKB

NP_034682.1 - NCBI Protein

16171 - Gene ID

Application Details

Tested Applications: ELISA, FC, WB, Multiplex

Application Note: IL-17 A antibody has been tested for use in western blotting, Flow Cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.

Formulation

Concentration: 2.94 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store IL-17A antibody at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and mouse IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Flow Cytometry (FC) Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.