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MIF Antibody
Chicken Polyclonal
200-901-457
500 µg
Lyophilized
WB, ELISA
Human
Chicken
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Macrophage migration inhibitory factor (MIF) (CHICKEN) Antibody - 200-901-457
chicken Anti-Macrophage migration inhibitory factor antibody, chicken Anti-MIF antibody, Phenylpyruvate tautomerase antibody, GIF antibody, GLIF antibody, Glycosylation inhibiting factor antibody, Macrophage migration inhibitory factor antibody, MIF protein antibody
Chicken
Polyclonal
IgY
Target Details
MIF - View All MIF Products
Human
Conjugated Peptide
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the Fc domain of the chicken IgG molecule may interact with cells non-specifically.
Application Details
ELISA, WB
This IgY fraction antibody of anti-Human macrophage migration inhibitory factor (MIF) has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry and flow cytometry. The antibody recognizes 12,300 MW mature human MIF. The MIF gene encodes a protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown.
Formulation
5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
500 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine. It is involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. It counteracts the anti-inflammatory activity of glucocorticoids. MIF has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.