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Microtubule Associated Protein 2 (MAP2) Antibody
Chicken Polyclonal
200-901-D68
100 µL
Liquid
WB, IHC
Human, Mouse, Rat, Bovine
Chicken
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Microtubule Associated Protein 2 (MAP2) (Chicken) Antibody - 200-901-D68
Microtubule-associated protein 2, MAP2, MAP-2,Map2, Map-2
Chicken
Polyclonal
IgY
Target Details
MAP2 - View All MAP2 Products
Human, Mouse, Rat, Bovine
Native Protein
Anti-Microtubule Associated Protein 2 Antibody was produced in chicken by repeated immunizations with recombinant bovine MAP2 protein expressed in and purified from E. Coli.
Anti-MAP2 antibody is directed against MAP2 protein. The antibody is a total IgY fraction. The antibody has been directly tested for reactivity in bovine, human, mouse and rat tissues. It is expected that the antibody will react with other mammalian tissues. Cross reactivity with MAP2 from other species has not been determined.
P11137 - UniProtKB
DAA32510.1 - NCBI Protein
Application Details
IHC, WB
Anti-Microtubule Associated Protein 2 antibody is tested for use in Western Blotting, ICC, and IHC. Specific conditions should be optimized by the end user. Expect a band of approximately 280 kDa in size corresponding to MAP2 proteins in Western blots in the appropriate cell lysate or extract.
Formulation
Titrated value sufficient to run approximately 10 mini blots. by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
MAP2 antibody detects Microtubule Associated Protein 2. Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.