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Mcl-1 Antibody
Rabbit Polyclonal
2 References
200-401-CR9
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Mcl-1 (RABBIT) Antibody - 200-401-CR9
Mcl-1 Antibody, TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3
Rabbit
Polyclonal
IgG
Target Details
MCL1 - View All MCL1 Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Mcl-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 16 amino acids near the C-terminus of human Mcl-1.
Anti-Mcl-1 Antibody is affinity chromatography purified via peptide column. Detects isoforms Mcl-1L and Mcl-1ES.
Application Details
ELISA, IF, IHC, WB
Anti-Mcl-1 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 37 kDa in Western Blots of specific cell lysates and tissues.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.
Adams et al. (2017). BCL-W has a fundamental role in B cell survival and lymphomagenesis. Journal of Clinical Investigation
Applications
WB, IB, PCA
Choudhary, GS et al. (2015). Cyclin E/Cdk2-dependent phosphorylation of Mcl-1 determines its stability and cellular sensitivity to BH3 mimetics. Oncotarget
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
