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Mash1/Ascl1 Antibody
Goat Polyclonal
600-101-MH4S
600-101-MH4
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, IHC
Human, Mouse
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Mash1/Ascl1 (GOAT) Antibody - 600-101-MH4
Goat Anti-Ascl1 Antibody, Goat Anti-Mash1 Antibody, Achaete-scute homolog 1, ASH-1, mASH-1
Goat
Polyclonal
IgG
Target Details
Ascl1 - View All Ascl1 Products
Human, Mouse
Conjugated Peptide
Anti-Mash1/Ascl1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Mash1/Ascl1 conjugated to Keyhole Limpet Hemocyanin (KLH).
This affinity purified antibody is directed against mouse Mash1/Ascl1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and rat, and 93% homology with human.
Application Details
IHC, WB
Anti-Mash1/Ascl1 Antibody has been tested in Western Blot and Immunohistochemistry. Expect a band at ~24.7 kDa in western blot using appropriate lysates or tissues. Positive control used was SH-SY5Y whole cell lysate in WB, human thyroid papillary carcinoma tissue in IHC.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Ascl1 (Achaete-scute homolog 1) is a transcription factor that plays a key role in neuronal differentiation, it acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. It directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Ascl1 plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. It is essential for the generation of olfactory and autonomic neurons. It acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Anti-Mash1/Ascl1 Antibody is useful for researchers interested in CCHS, SIDS, Dopaminergic Neurogenesis, and DNA binding.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.