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MAPKAP Kinase 2 Antibody
Rabbit Polyclonal
600-401-281
100 µg
Liquid (sterile filtered)
WB
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-MAPKAP Kinase 2 (RABBIT) Antibody - 600-401-281
rabbit anti-MAPKAP kinase 2 antibody, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase-2, MK2, mitogen-activated protein kinase-activated protein kinase-2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2
Rabbit
Polyclonal
IgG
Target Details
MAPKAPK2 - View All MAPKAPK2 Products
Human
Conjugated Peptide
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
This affinity-purified antibody is directed against rabbit MAPKAP Kinase 2 and is useful in determining its presence in various assays. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish. Cross reactivity with MAPKAP Kinase 2 from other sources has not been tested.
Application Details
WB
Anti-MAPKAP kinase 2 antibody is tested by western blotting and suitable for ELISA, immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. The antibody detects a single 44 kDa protein in crude extracts prepared either from unstimulated (p/n w09-000-364) or TNFα stimulated ((p/n w09-000-367) human HeLa cell lysates. A 1:15,000 dilution is recommended for ELISA. The researcher should determine optimal titers for other applications.
Formulation
0.9 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The best-characterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNFα at a post-transcriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.