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Luciferase Antibody Peroxidase Conjugated
Goat Polyclonal
4 References
200-103-150S
200-103-150-0100
200-103-150
25 µL
100 µL
20 mg
Liquid (sterile filtered)
Lyophilized
Lyophilized
WB, ELISA, IHC
Luciferase
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Luciferase (GOAT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-103-150
goat anti-Luciferase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Luciferase Antibody
Goat
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Luciferase
Native Protein
Luciferase [Photinus pyralis (Firefly)]
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Application Details
ELISA, WB
IHC
- View References
Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Anti-Luciferase Peroxidase has been tested by ELISA and western blot. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 ug of Luciferase [Photinus pyralis (Firefly)] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.
Hu L et al. (2023). A first-in-class inhibitor of Hsp110 molecular chaperones of pathogenic fungi. Nat Commun.
Applications
WB, IB, PCA
Rao L et al. (2022). Glutamate catabolism during sporulation determines the success of the future spore germination. iScience.
Applications
WB, IB, PCA
Parrell D et al. (2020). Channels modestly impact compartment-specific ATP levels during Bacillus subtilis sporulation and a rise in the mother cell ATP level is not necessary for Pro-σ K cleavage. Mol Microbiol.
Applications
WB, IB, PCA
Lina IA et al. (2019). A mouse model for the study of transplanted bone marrow mesenchymal stem cell survival and proliferation in lumbar spinal fusion. Eur Spine J.
Applications
IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.