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Datasheet

Luciferase Antibody Fluorescein Conjugated

Goat Polyclonal

200-102-150S 200-102-150-0100 200-102-150
25 µL 100 µg 20 mg
Liquid (sterile filtered) Lyophilized Lyophilized
WB, Dot Blot
Luciferase
Goat
$125.00 /Per Item
$445.00 /Per Item
$3,549.00 /Per Item
25 µL $125.00
100 µg $445.00
20 mg $3,549.00
In stock
Ships next business day
In stock
Ships next business day
In stock
Ships next business day
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Anti-Luciferase (GOAT) Antibody Fluorescein Conjugated (BULK ORDER) - 200-102-150
goat anti-Luciferase Antibody FITC Conjugation, Fluorescein Conjugated goat anti-Luciferase Antibody
Goat
Fluorescein (FITC)
Polyclonal
IgG

Target Details

Luciferase
Native Protein
Luciferase (Photinus pyralis (Firefly))
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum and purified and partially purified Luciferase (Photinus pyralis (Firefly)).

Application Details

Dot Blot, WB
Anti-Luciferase Conjugated Antibody has been tested by western blot and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.