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Datasheet

LIMA1 Antibody

Rabbit Polyclonal

600-401-CK6
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human
Rabbit
$475.00 /Per Item
Out of stock
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Product Details

Anti-LIMA1 (RABBIT) Antibody - 600-401-CK6
LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3
Rabbit
Polyclonal
IgG

Target Details

LIMA1  - View All LIMA1 Products
Human
Conjugated Peptide
Anti-LIMA1 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the N-terminus of human LIMA1
Anti-LIMA1 antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Q9UHB6 - UniProtKB
NP_001107018 - NCBI Protein
51474 - Gene ID

Application Details

ELISA, IF, IHC, WB
Anti-LIMA1 Antibody has been tested for use in ELISA, Immunohistochemistry, and Immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 85 kDa in Western Blots of specific cell lysates and tissues.

Formulation

1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.