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KLH Antibody
Mouse Monoclonal 3B1 IgM
200-301-E66S
200-301-E66
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
KLH
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-KLH (MOUSE) Monoclonal Antibody - 200-301-E66
mouse anti-KLH Antibody, Keyhole Limpet Hemocyanin, KLH antibody
Mouse
Monoclonal
IgM
Target Details
KLH
Native Protein
Anti-KLH (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.
Anti-KLH is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. KLH antibody is specific for KLH. Does not cross react with mouse heart or skin tissue sections. Cross-reactivity from other sources has not been determined.
Application Details
Anti-KLH can be used as an IgM isotype control in various assays or in ELISA assays to detect KLH or KLH conjugates. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
0.1% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store KLH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
KLH antibody is used to detect Keyhole Limpet Hemocyanin. KLH is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.